Du
01 May 2008
au
31 December 2012

CONffIDENCE

FP7 EU collaborative project, partner

Context

RASFF, the rapid alert system on food and feed shows that controls on chemical contaminants in food and feed are essential for food safety in Europe. Chemical contaminants are also a major consumer concern in terms of dietary risks. Moreover the General Food Law regulation (Regulation (EC)178/2002), makes industries responsible for the safety of their products. Current analytical methods are often expensive and can detect only one substance at a time. There is thus an urgent need to develop screening methods which are straightforward, inexpensive and rapid and that can detect several contaminants at the same time. In this context, a 56 months European collaborative project (CP) funded by the 7th Framework Programme has been launched in May 2008. This project CONffIDENCE (proposal-211326) was entitled "Contaminants in Food and Feed: Inexpensive Detection for Control of Exposure".

Objectives

The strategic objectives from the CONffIDENCE project are: 
1° Develop new simplified inexpensive detection methods for chemical contaminants from farm to fork in order to assure chemical safety and quality in the European food supply;
2° Develop new detection tools for EFSA recognised key and emerging risks;
3° Improve the exposure assessment based on a better idea about average contaminant levels thanks to increased monitoring data by simplified and more cost-efficient detection tools;
4° Contribute to the validation of risk benefit and predictive hazard behaviour models in accordance with the strategic agenda of the European Technology Platform (ETP) Food for Life;
5° Disseminate the new detection methods to all relevant stakeholders, including industrial and governmental end-users as well as students.

Expected results

The project aimed to provide long-term solutions to the monitoring of persistent organic pollutants (POPs), perfluorinated compounds (PFCs), pesticides, veterinary pharmaceuticals (coccidiostats, antibiotics), heavy metals and biotoxins (alkaloids, plant contaminant, marine toxins, mycotoxins) in high-risk products like fish and fish feed, cereal-based food/feed and vegetables. Several new methods were developed, based on novel multiplex technologies such as dipsticks, flow cytometry with functionalised beads, surface plasmon resonance optical (SPR) and electrochemical biosensors, cytosensors or near infrared hyperspectral imaging. After validation, the new methods were applied in demonstration activities which helped to assess contaminants exposure and validate the risk models. Dissemination to scientists and to relevant stakeholders, including the food and feed industry, regulatory control (DG-SANCO, EFSA) and normalisation bodies (CEN), the Community reference laboratories (CRLs), the routine laboratories and the consumers were assured by a website, an electronic Newsletter, press articles, public workshops, open days, lectures at international conferences, publications and training modules.

Results obtained

All these results can be found in the section “Project output” of the CONffIDENCE website (http://www.conffidence.eu ). In particular, regarding the CRA-W contribution; the protocol and the validation of the NIR hyperspectral imaging method for the detection and quantification of ergot contamination in cereals dedicated for food and feed has been described in detail and published (Food Additives and Contaminants, 2012). The decision tool is based on chemometics discrimination models and morphological identification rules applied on the NIR hyperspectral images. The hyperspectral imaging method has been successfully rounded off with testing and demonstrating the system at an industrial site (see figure here below). The validation and the transferability of this methodology have been presented and demonstrated at several events and opendays organized in the framework of the CONffIDENCE project and will be shortly published in a special issue of the Analytical and Bioanalytical Chemistry journal dedicated to the outputs of the project. This technology can be used to detect and quantify ergot contamination in large samples of cereals in both the laboratory and in the processing industry, where accurate and fast inspection is needed. The classical microscopy method can be used as confirmatory method on reduced samples. The line scan NIR hyperspectral imaging system allows to analyse a sample of 250 g in one minute. In comparison, the existing microscopy method requires 30-60 minutes to analyse the same sample. The next challenge will be to demonstrate the potential of the methodology for multicontaminants detection in particular in the organic productions.

Contribution

The CRA-W led the workpackage on training, dissemination and exploitation of the results including the development of the website. The CRA-W was also member of the project management board and was involved in the project management by setting up and maintaining the CONffIDENCE intranet. Moreover, CRA-W played a key role in the development of a near infrared imaging based analytical method for detection of ergot (claviceps purpurea) in cereals. Ergot was selected because of its increasing presence in recent years in samples of cereals or compound feeds.

Partners

The CONffIDENCE project was coordinated by RIKILT. The consortium consists of 17 partners from 10 countries, representing 9 research institutes, 5 universities, 2 large food and feed industries and 1 SMEs.
- RIKILT-Institute of food safety (NL)
- ICT-Institute of Chemical Technology (CZ)
- CSIC- National Council of Scientific Research (SP)
- CVUA - Chemisches und Veterinäruntersuchungsamt (DE)
- EC-JRC-IRMM-European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (BE)
- CSL-Central Science Laboratory (UK)
- DTU-Food-Technical University of Denmark (DK)
- RIVM- National Institute for Public Health and the Environment (NL)
- QUB- Queen's University Belfast (UK)
- ISPA-Institute of Sciences of Food Production (IT)
- CRA-W- Walloon Agricultural Research Centre (BE)
- TUT-Tampere University of Technology (FI)
- NUTRECO Holding N.V. (NL)
- NESTLE (CH)
- USC- University of Santiago de Compostelle (SP)
- CER-Centre d'Economie Rurale (BE)
- UNISENSOR (BE)

CRAW off coordinator

Jacob de Jong, RIKILT - Institute of Food Safety, Wageningen, the Netherlands

Funding

  • CE - DG Research - FP7

Publications

Vermeulen, P. , Fernández Pierna, J.A. , Dardenne, P. & Baeten, V. (2011). CONffIDENCE: Detection of contaminants in cereals by near infrared hyperspectral imaging. Poster in: RAFA 2011 Conference, Prague, November 2011. Vermeulen, P. , Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2009). Contribution to the FP7 - CONffIDENCE project of near infrared hyperspectral imaging: detection of contaminants. Poster in: 3rd International Feed Safety Conference 2009: Methods and Challenges, Wageningen - The Netherlands, 6-7 October 2009. Vermeulen, P. , Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2009). Detection of ergot bodies in cereals by hyperspectral near infrared imaging. Poster in: International workshop on multivariate image analysis, Valencia - Spain, 28-29 September 2009. Vermeulen, P. , Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2010). Detection of ergot bodies in cereals by hyperspectral NIR imaging. Poster in: IASIM 2010, Dublin - Ireland, 18-19 November 2010. Vermeulen, P. , Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2011). Detection of ergot bodies in cereals by near infrared spectroscopy and hyperspectral near infrared imaging. Proceedings in: 14th International Conference on Near Infrared Spectroscopy (ICNIRS): Breaking the dawn, Bangkok - Thailand, 7-13 November 2009, 997-1002. Vermeulen, P. , Sinnaeve, G. , Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2010). Detection of ergot bodies in cereals by NIRS and hyperspectral NIR imaging. Poster in: CONffIDENCE: 1st Open Day, Noordwijkerhout - The Netherlands, 27 January 2010. Vermeulen, P. , Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2009). Detection of ergot bodies in cereals by NIRS and hyperspectral NIR imaging. Poster in: 14th International Conference on Near Infrared Spectroscopy (ICNIRS): Breaking the dawn, Bangkok - Thailand, 7-13 November 2009. Ebene, M.B. (2016). Detection par technique vibrationnelle de l'ergot de seigle dans les farines à destination humaine. Louvain-la Neuve, UCL, Mémoire, 124. Vermeulen, P. , Ebene, M.B. , Fernández Pierna, J.A. , Lecler, B. , Veys, P. & Baeten, V. (2016). Development of near infrared spectroscopy methods for the detection of ergot in cereal flour. Poster in: Feed 2016: 5th International Feed Conference: Present and Future Challenges, Geel - Belgium, 19 - 20 October 2016. Vermeulen, P. , Fernández Pierna, J.A. , Van Egmond, H.P. , Zegers, J. , Dardenne, P. & Baeten, V. (2012). Near infrared hyperspectral imaging methodology as a control tool for the detection of ergot in cereals. Poster in: IUPAC, Rotterdam, 8 November 2012. Vermeulen, P. , Burger, J. & Fernández Pierna, J.A. (2010). NIR Hyperspectral Imaging and chemometrics as a lab tool for the quality control of agricultural products. Poster in: Chemometrics in analytical chemistry (CAC 2010), Antwerp - Belgium, 18-21 October 2010. Baeten, V. , Vermeulen, P. , Dardenne, P. & Fernández Pierna, J.A. (2010). NIR hyperspectral imaging methods for quality and safety control of food and feed products: contributions to four European projects NIR News 21, (6), 10-13. Fernández Pierna, J.A. , Vermeulen, P. , Amand, O. , Tossens, A.. , Dardenne, P. & Baeten, V. (2012). NIR hyperspectral imaging spectroscopy and chemometrics for the detection of undesirable substances in food and feed. Chemometrics and Intelligent Laboratory Systems, 117: 233-239. Vermeulen, P. , Fernández Pierna, J.A. , Dardenne, P. & Baeten, V. (2011). On-line detection and quantification of ergot bodies in cereals by near infrared hyperspectral imaging. Poster in: 15th International Conference on Near Infrared Spectroscopy (ICNIRS): NIR at the end of the rainbow, Cape Town - South Africa, 13-20 May 2011. Vermeulen, P. , Fernández Pierna, J.A. , Van Egmond, H.P. , Dardenne, P. & Baeten, V. (2012). On-line detection and quantification of ergot bodies in cereals using near infrared hyperspectral imaging. Food Additives and Contaminants: Part A, 29: (2), 232-240. Vermeulen, P. , Ebene, M.B. , Orlando, B. , Fernández Pierna, J.A. & Baeten, V. (2017). Online detection and quantification of particles of ergot bodies in cereal flour using near infrared hyperspectral imaging. Food Additives & Contaminants: Part A, 34: (8), 1312-1319. Vermeulen, P. & Baeten, V. (2008). Premier projet FP7 pour le CRA-W CRA-W info 19, p1. Vermeulen, P. & De Jong, J. (2009). Safer food through rapid tests for chemical contaminants Food Science & Technology 23, (3), 26-28. Vermeulen, P. , Fernández Pierna, J.A. , Van Egmond, H.P. , Zegers, J. , Dardenne, P. & Baeten, V. (2013). Transfer of methodology from lab to industry for the detection of ergot. Proceedings in: RAFA 2013: workshop on Infrared spectroscopy, Raman spectroscopy and chemometrics for monitoring of food and feed products, lab-to-the-sample, Prague, Czech Republic, 5 November 2013, 27. Vermeulen, P. , Fernández Pierna, J.A. , Van Egmond, H.P. , Zegers, J. , Dardenne, P. & Baeten, V. (2013). Validation and transferability study of a method based on near-infrared hyperspectral imaging for the detection and quantification of ergot bodies in cereals. Analytical and Bioanalytical Chemistry, 405: (24), 7765-7772. Vermeulen, P. , Fernández Pierna, J.A. , Abbas, O. , Dardenne, P. & Baeten, V. (2011). Vibrational spectroscopy for the authentication and traceability of food products: contributions to european projects. Poster in: ASSET 2011: Food integrity and traceability conference, Belfast - UK, 21-24 March 2011.