DMI fungicide resistance in Zymoseptoria tritici is unlinked to geographical origin and genetic background: a case study in Europe


  • Oreiro, Eula Gems , Samils, Berit , Kildea, Steven , Heick, Thies , Hellin, Pierre , Legrève, Anne , Rodemann, Bernd , Berg, Gunilla , Jørgensen, Lise N , Friberg, Hanna , Berlin, Anna , Zhan, Jiasui & Andersson, Björn (2024). DMI fungicide resistance in Zymoseptoria tritici is unlinked to geographical origin and genetic background: a case study in Europe. Pest Management Science,
Type Journal Article
Year 2024
Title DMI fungicide resistance in Zymoseptoria tritici is unlinked to geographical origin and genetic background: a case study in Europe
Journal Pest Management Science
Project/Service ref RESIST / EURORES
Abstract BACKGROUND: The hemibiotrophic fungus Zymoseptoria tritici causing Septoria tritici blotch (STB), is a devastating foliar path- ogen of wheat worldwide. A common group of fungicides used to control STB are the demethylation inhibitors (DMIs). DMI fun- gicides restrict fungal growth by inhibiting the sterol 14-⊍-demethylase, a protein encoded by CYP51 gene and essential for maintaining fungal cell permeability. However, the adaptation of Z. tritici populations in response to intensive and prolonged DMI usage has resulted in a gradual shift towards reduced sensitivity to this group of fungicides. In this study, 311 isolates were collected pre-treatment from nine wheat-growing regions in Europe in 2019. These isolates were analysed by high-throughput amplicon-based sequencing of nine housekeeping genes and the CYP51 gene. RESULTS: Analyses based on housekeeping genes and the CYP51 gene revealed a lack of population structure in Z. tritici sam- ples irrespective of geographical origin. Minimum spanning network (MSN) analysis showed clustering of multilocus genotypes (MLGs) based on CYP51 haplotypes, indicating an effect of selection due to DMI fungicide use. The majority of the haplotypes identified in this study have been reported previously. The diversity and frequencies of mutations varied across regions. CONCLUSION: Using a high-throughput amplicon-sequencing approach, we found several mutations in the CYP51 gene com- bined in different haplotypes that are likely to cause fungicide resistance. These mutations occurred irrespective of genetic background or geographical origin. Overall, these results contribute to the development of effective and sustainable risk mon- itoring for DMI fungicide resistance.
Fichier
Authors Oreiro, Eula Gems, Samils, Berit, Kildea, Steven, Heick, Thies, Hellin, Pierre, Legrève, Anne, Rodemann, Bernd, Berg, Gunilla, Jørgensen, Lise N, Friberg, Hanna, Berlin, Anna, Zhan, Jiasui, Andersson, Björn