Physiological responses and the expression of sugar associated genes in faba bean (Vicia faba L.) exposed to osmotic stress

Ghouili, E. , Sassi, K. , Jebara, M. , Hidri, Y. , Ouertani, R. , Muhovski, Y. , Jebara, S. , El Ayed, M. , Abdelkarim, S. , Chaieb, O. , Jallouili, S. , Kalleli, F. , Mhamdi, M. , Souissi, F. & Abid, G. (2021). Physiological responses and the expression of sugar associated genes in faba bean (Vicia faba L.) exposed to osmotic stress. Physiol Mol Biol Plants, 27: (1), 135–150.

Type	Journal Article
Year	2021
Title	Physiological responses and the expression of sugar associated genes in faba bean (Vicia faba L.) exposed to osmotic stress
Journal	Physiol Mol Biol Plants
Label	U1-Muhovski
Volume	27
Issue	1
Pages	135–150
Date	January 2021
Endnote Keywords	Faba bean, Gene expression, GC-MS, Osmotic stress, Sugar metabolism, qRT-PCR
Abstract	Faba bean (Vicia faba L.) is the major food legume crop in Tunisia. However, its growth and yield is strongly affected by water‐limited environments. In this study, osmotic stress exhibited a negative effect on Bachar and Badii cultivar. Nevertheless, the deteriorating effects of osmotic stress were relatively low on studied parameters of Bachar due to its better efficiency to reduce oxidative damage by increasing enzymatic activities such as catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX), accumulation of total chlorophyll (Chlt), soluble sugars and leaf relative water content (RWC). GC–MS analysis determined a total of 11 soluble carbohydrates induced by osmotic stress and differentially accumulated in the both cultivars. Bachar showed elevated levels of mannose, glucose, galactose, ribose, rhamnose and myo-inositol which might help to maintain osmotic adjustment, membranes and proteins protection from the damaging effect of reactive oxygen species. Sugar metabolism related genes (VfNINV3, VfPHS2, VfFRK4, VfHXK1, VfGPI1, VfSTP1.1, VfpGlcT1.1, VfSTP5.1, VfpGlcT1.2, VfSWEET2.1, VfVINV2, VfSUS1, VfPGM1, VfSUT1.1, VfGPT1, VfSPS1, VfSPP1, VfPHS1, VfSUT4.1 and VfTMT1.1) were differentially expressed in both cultivars demonstrating their important roles in sugar accumulation. Most of these genes were upregulated in the leaves of Bachar under moderate and severe stress, which could lead to increase glycolysis and tricarboxylic acid cycle in order to accelerate energy production, necessary to increase osmotic regulation and consequently enhancing the osmotic stress tolerance in that cultivar. Overall, sugars accumulation ability can be used as a useful indicator for the osmotic stress tolerant potential in faba bean breeding programs.
Fichier	Physiological responses and the expression of sugar associated genes in faba bean (Vicia faba L.) exposed to osmotic stress
Lien	https://doi.org/10.1007/s12298-021-00935-1
Authors	Ghouili, E., Sassi, K., Jebara, M., Hidri, Y., Ouertani, R., Muhovski, Y., Jebara, S., El Ayed, M., Abdelkarim, S., Chaieb, O., Jallouili, S., Kalleli, F., Mhamdi, M., Souissi, F., Abid, G.

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